MINISTRY OF AGRICULTURE AND RURAL DEVELOPMENT -------	THE SOCIALIST REPUBLIC OF VIETNAM Independence - Freedom - Happiness ---------------
No. 07/2019/TT-BNNPTNT	Hanoi, August 07, 2019

 

CIRCULAR

PROMULGATING NATIONAL TECHNICAL REGULATION ON AQUACULTURE FEED

Pursuant to the Law on Promulgation of Legislative Documents dated June 22, 2015;

Pursuant to the Law on Standards and Technical Regulations dated June 29, 2006;

Pursuant to the Law on Fisheries dated November 21, 2017;

Pursuant to the Government’s Decree No. 15/2017/ND-CP dated February 17, 2017 defining the functions, tasks, powers and organizational structure of the Ministry of Agriculture and Rural Development;

Pursuant to the Government’s Decree No. 26/2019/ND-CP dated March 08, 2019 on guidelines for implementation of the Law on Fisheries;

At the request of the Director of the Department of Science, Technology and Environment and the Director General of the Directorate of Fisheries;

The Minister of Agriculture and Rural Development hereby promulgates National technical regulation on aquaculture feed.

Article 1. The National technical regulation on aquaculture feed is promulgated together with this Circular.

1. Aquaculture feed. Part 1: Compound feeds

Code: QCVN 02-31-1 : 2019/BNNPTNT.

2. Aquaculture feed. Part 2: Feed supplements

Code: QCVN 02-31-2 : 2019/BNNPTNT.

3. Aquaculture feed. Part 2: Fresh and live feeds

Code: QCVN 02-31-3 : 2019/BNNPTNT.

Article 2. This Circular comes into force from January 01, 2020.

Article 3. Chief of the Office of the Ministry of Agriculture and Rural Development; Director of Department of Science, Technology and Environment; Director General of Directorate of Fisheries; heads of affiliates of the Ministry of Agriculture and Rural Development; Directors of Departments of Agriculture and Rural Development of provinces and central-affiliated cities and relevant organizations and individuals are responsible for the implementation of this Circular.

Difficulties that arise during the implementation of this Circular should be promptly reported to the Ministry of Agriculture and Rural Development for its possible review or revision./.

 

PP. THE MINSITER THE DEPUTY MINISTER Phung Duc Tien

 

QCVN 02 - 31 - 1: 2019/BNNPTNT

NATIONAL TECHNICAL REGULATION AQUACULTURE FEED

PART 1: COMPOUND FEEDS

 

Foreword

QCVN 02 - 31 - 1 : 2019/BNNPTNT is developed by Vietnam National University of Agriculture, presented by the Directorate of Fisheries and promulgated by the Minister of Agriculture and Rural Development together with the Circular No. 07/2019/TT-BNNPTNT dated August 07, 2019.

 

NATIONAL TECHNICAL REGULATION AQUACULTURE FEED

PART 1: COMPOUND FEEDS

1. GENERAL

1.1. Scope

This document provides for safety criteria and maximum levels in compound feeds (HS code 2309.90.13; 2309.90.19) intended for use in aquaculture.

1.2. Regulated entities

This document applies to organizations and individuals involved in manufacturing and import of compound feeds intended for use in aquaculture in Vietnam.

1.3. Normative references

AOAC 986.15, Arsenic, cadmium, lead, selenium and zinc in human and pet foods by atomic absorption spectrometry (AAS) and anodic stripping voltammetry (ASV).

EN 16277:2012, Animal feeding stuffs - Determination of mercury by cold- vapour atomic absorption spectrometry (CVAAS) after microwave pressure digestion (extraction with 65 % nitric acid and 30 % hydrogen peroxide).

EN 16278:2012, Animal feeding stuffs - Determination of inorganic arsenic by hydride generation atomic absorption spectrometry (HG-AAS) after microwave extraction and separation by solid phase extraction (SPE).

TCVN 4325:2007 (ISO 6497:2002), Animal feeding stuffs – Sampling.

TCVN 6952:2001 (ISO 9495:1998), Animal feeding stuffs – Preparation of test samples.

TCVN 6953:2001 (ISO 14718:1998), Animal feeding stuffs - Determination of aflatoxin B1 content of mixed feeding stuffs - Method using high-performance liquid chromatography.

TCVN 9126: 2011 (ISO 17375:2006), Animal feeding stuffs - Determination aflatoxin B1. Method using high-performance liquid chromatography with post-column derivatization.

TCVN 9588:2013 (ISO 27085:2009), Animal feeding stuffs - Determination of calcium, sodium, phosphorus, magnesium, potassium, iron, zinc, copper, manganese, cobalt, molybdenum, arsenic, lead and cadmium by ICP-AES (ICP-AES).

TCVN 10780-1:2017 (ISO 6579-1:2017), Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.

TCVN 11282:2016, Animal feeding stuffs -Determination of ethoxyquin content- Fluorometric method.

TCVN 11283:2016 (AOAC 996.13), Animal feeding stuffs - Determination of ethoxyquin content - Liquid chromatographic method.

TCVN 11923:2017 (ISO/TS 17728:2015), Microbiology of the food chain - Sampling techniques for microbiological analysis of food and feed samples.

In the cases where new regulations on sampling, preparation of test samples and test methods are promulgated or old ones specified in this document are amended or replaced, the newest ones shall apply.

2. TECHNICAL RRESCRIPTION

Maximum levels for safety criteria

No.	Criterion	Unit	Maximum level
1	Aflatoxin B1	µg/kg	10
2	Ethoxyquin	mg/kg	150
3	Lead (Pb)	mg/kg	5
4	Cadmium (Cd)	mg/kg	1
5	Mercury (Hg)	mg/kg	0.4
6	Inorganic arsenic (As)	mg/kg	2
7	Salmonella	CFU/25g	Not detected

3. TEST METHODS

3.1. Sampling

TCVN 11923:2017 (ISO/TS 17728:2015): for samples intended for microbiological analysis

TCVN 4325:2007 (ISO 6497:2002): for samples intended for analysis of other criteria

3.2. Preparation of test samples

TCVN 6952:2001 (ISO 14718:1998) is applied.

3.3. Test methods

No.	Criterion	Test method
1	Aflatoxin B1	TCVN 6953:2001 (ISO 14718:1998) TCVN 9126:2011 (ISO 17375:2006)
2	Ethoxyquin	TCVN 11282:2016 TCVN 11283:2016 (AOAC 996.13)
3	Lead (Pb)	AOAC 986.15 TCVN 9588:2013 (ISO 27085:2009)
4	Cadmium (Cd)	AOAC 986.15 TCVN 9588:2013 (ISO 27085:2009)
5	Mercury (Hg)	EN 16277:2012
6	Inorganic arsenic (As)	EN 16278:2012 TCVN 9588:2013 (ISO 27085:2009)
7	Salmonella	TCVN 10780-1:2017 (ISO 6579-1:2017)

4. MANAGEMENT REGULATION

4.1. Declaration of conformity

Organizations and individuals shall submit declarations of conformity of compound feeds as follows:

4.1.1. Regarding domestically manufactured products, the certification result given by the certification body registered or accredited as prescribed by law shall be sent.

4.1.2. Regarding imported products, the certification or assessment result given by the certification body or the conformity assessment body registered or accredited as prescribed by law shall be sent.

4.2. Conformity assessment

Organizations and individuals shall assess conformity of compound feeds as follows:

4.2.1. Regarding domestically manufactured products, Method 1 (Representative form testing) shall be adopted.

4.2.2. Regarding imported products, Method 7 (Testing and evaluation of batches of goods) shall be adopted.

4.3. Procedures and documentation requirements for declaration of conformity are specified in Clauses 3 and 4 Article 1 of the Circular No. 02/2017/TT-BKHCN dated March 31, 2017 of the Ministry of Science and Technology on amendments to the Circular No. 28/2012/TT-BKHCN dated December 12, 2012.

Departments of Agriculture and Rural Development of provinces and central-affiliated cities shall receive documents submitted for declaration of conformity by organizations and individuals registering their manufacturing and trading within provinces and cities.

4.4. Contents, sequence and principles of using conformity assessment methods are specified in the Appendix II of the Circular No. 28/2012/TT-BKHCN dated December 12, 2012 of the Ministry of Science and Technology.

5. RESPONSIBILITIES OF ORGANIZATIONS AND INDIVIDUALS

Organizations and individuals mentioned in Section 1.2 shall comply with regulations of this document.

6. IMPLEMENTATION

6.1. The Directorate of Fisheries and Departments of Agriculture and Rural Development of provinces and cities shall inspect the implementation of this document as assigned by the Ministry of Agriculture and Rural Development.

6.2. The Directorate of Fisheries shall disseminate, provide guidance and cooperate with relevant authorities in organizing the implementation of this document.

6.3. In the cases where any of the regulations specified in this document is amended or replaced, the newest one shall apply.

 

QCVN 02 - 31 - 2 : 2019/BNNPTNT

NATIONAL TECHNICAL REGULATION AQUACULTURE FEED

PART 2: FEED SUPPLEMENTS

 

Foreword

QCVN 02 - 31 - 1 : 2019/BNNPTNT is developed by Research Institute for Aquaculture No. 2, presented by the Directorate of Fisheries and promulgated by the Minister of Agriculture and Rural Development together with the Circular No. 07/2019/TT-BNNPTNT dated August 07, 2019.

 

NATIONAL TECHNICAL REGULATION AQUACULTURE FEED

PART 2: FEED SUPPLEMENTS

1. GENERAL

1.1. Scope

This document provides for safety criteria and maximum levels in feed supplements (HS code 2309.90.20) intended for use in aquaculture.

1.2. Regulated entities

This document applies to organizations and individuals involved in manufacturing and import of feed supplements intended for use in aquaculture in Vietnam.

1.3. Normative references

AOAC 986.15, Arsenic, cadmium, lead, selenium and zinc in human and pet foods by atomic absorption spectrometry (AAS) and anodic stripping voltammetry (ASV).

EN 16277:2012, Animal feeding stuffs - Determination of mercury by cold- vapour atomic absorption spectrometry (CVAAS) after microwave pressure digestion (extraction with 65 % nitric acid and 30 % hydrogen peroxide).

EN 16278:2012, Animal feeding stuffs - Determination of inorganic arsenic by hydride generation atomic absorption spectrometry (HG-AAS) after microwave extraction and separation by solid phase extraction (SPE).

TCVN 4325: 2007 (ISO 6497:2002), Animal feeding stuffs – Sampling.

TCVN 6952: 2001 (ISO 9498:1998), Animal feeding stuffs – Preparation of test samples.

TCVN 7407:2004 (AOAC 991.31), Cereals, pulses and oil seeds - Determination of aflatoxins by immunoaffinity column method.

TCVN 7924 - 2: 2008, Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli - Part 2: Colony-count technique at 44°C using 5-bromo-4-chloro-3-indolyl β-D-glucuronide.

TCVN 9126: 2011 (ISO 17375:2006), Animal feeding stuffs - Determination of aflatoxin B1. Method using high-performance liquid chromatography with post-column derivatization.

TCVN 9588: 2013 (ISO 27085:2009), Animal feeding stuffs - Determination of calcium, sodium, phosphorus, magnesium, potassium, iron, zinc, copper, manganese, cobalt, molybdenum, arsenic, lead and cadmium by ICP-AES (ICP-AES).

TCVN 10780-1:2017 (ISO 6579-1:2017), Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.

TCVN 11283:2016 (AOAC 996.13), Animal feeding stuffs - Determination of ethoxyquin content - Liquid chromatographic method.

TCVN 11291:2016 (AOAC 957.22), Animal feeding stuffs - Determination of total arsenic content- Colorimetric method.

TCVN 11923:2017 (ISO/TS 17728:2015), Microbiology of the food chain - Sampling techniques for microbiological analysis of food and feed samples

In the cases where new regulations on sampling, preparation of samples and test methods are promulgated or old ones specified in this document are amended or replaced, the newest ones shall apply.

1.4. Interpretation of terms

For the purposes of this document, the terms below shall be construed as follows:

1.4.1. “feed supplement” means an ingredient or combination of ingredients added to a feed or diet to improve the nutritive balance or serve a specific purpose in aquaculture feeds.

1.4.2. “enzyme preparation” means a biological product containing one or more enzymes with or without a carrier.

1.4.3. “probiotic” means a biological product containing one or more strains of inactivated beneficial and live microorganisms with or without a carrier.

1.4.4. “prebiotic” means a biological product that includes nondigestible fibers (with or without a carrier) that stimulate the growth or activity of beneficial microorganisms in the tract.

1.4.5 “organic acid” means a feed containing one or more organic acids that play a beneficial role in improving digestion and/or promoting the health of animals or serving the purpose of feed preservation.

2. TECHNICAL PRESCRIPTION

2.1. Vitamins, amino acids, organic acids (single or combined)

Table 1- Maximum levels

No.	Criterion	Unit	Maximum level
Heavy metals
1	Inorganic arsenic (As)	mg/kg	2
2	Cadmium (Cd)	mg/kg	1
3	Lead (Pb)	mg/kg	5
4	Mercury (Hg)	mg/kg	0.2
Microorganisms
5	Escherichia coli	CFU/g	10
6	Salmonella	CFU/25g	Not detected.

2.2. Enzyme preparations, probiotics, prebiotics (single or combined)

Table 2- Maximum levels

No.	Criterion	Unit	Maximum level
Aflatoxin B1
1	Concentration of aflatoxin B1	µg/kg	10
Heavy metals
2	Cadmium (Cd)	mg/kg	3
3	Lead (Pb)	mg/kg	5
4	Mercury (Hg)	mg/kg	0.1
Microorganisms
5	Escherichia coli	CFU/g	10
6	Salmonella	CFU/25g	Not detected

2.3. Biologically active ingredients and products extracted from organisms

Table 3 - Maximum levels for biologically active ingredients and products extracted from plants

No.	Criterion	Unit		Maximum level
Heavy metals
1	Inorganic arsenic (As)	mg/kg		3
2	Lead (Pb)	mg/kg		2
3	Mercury (Hg)	mg/kg		1
Microorganisms
4	Escherichia coli		CFU/g	10
5	Salmonella		CFU/25g	Not detected

Table 4 - Maximum levels for biologically active ingredients and products extracted from animals

No.	Criterion	Unit	Maximum level
1	Ethoxyquin	mg/kg	150
Heavy metals
2	Total arsenic (As)*	mg/kg	10
3	Inorganic arsenic (As)	mg/kg	2
4	Cadmium (Cd)	mg/kg	2
5	Lead (Pb)	mg/kg	10
6	Mercury (Hg)	mg/kg	0.5
Microorganisms
6	Escherichia coli	CFU/g	10
7	Salmonella	CFU/25g	Not detected

* If the concentration of arsenic is less than 2 mg/kg, it is not required to test for inorganic arsenic

2.4. Minerals

Table 5- Maximum levels

No.	Criterion	Unit	Maximum level
Heavy metals
1	Inorganic arsenic (As)	mg/kg	12
2	Cadmium (Cd)	mg/kg	5
3	Lead (Pb)	mg/kg	30
4	Mercury (Hg)	mg/kg	0,2

2.5. Chemicals

Table 6- Maximum levels

No.	Criterion	Unit	Maximum level
Heavy metals
1	Inorganic arsenic (As)	mg/kg	2
2	Cadmium (Cd)	mg/kg	1
3	Lead (Pb)	mg/kg	5
4	Mercury (Hg)	mg/kg	1

3. TEST METHODS

3.1. Sampling

TCVN 11923:2017 (ISO/TS 17728:2015): for samples intended for microbiological analysis

TCVN 4325:2007 (ISO 6497:2002): for samples intended for analysis of other criteria

3.2. Preparation of test samples

TCVN 6952:2001 (ISO 9498:1998) is applied.

3.3. Test methods

No.	Criterion	Test method
1	Aflatoxin B1	TCVN 9126: 2011 (ISO 17375:2006) TCVN 7407:2004 (AOAC 991.31)
2	Total arsenic (As)*	TCVN 11291:2016(AOAC 957.22)AOAC 986.15
3	Inorganic arsenic (As)	EN 16278:2012 TCVN 9588:2013(ISO27085:2009)
6	Cadmium (Cd)	TCVN 9588:2013(ISO27085:2009) AOAC 986.15
7	Lead (Pb)	TCVN 9588:2013(ISO27085:2009) AOAC 986.15
8	Mercury (Hg)	EN 16277:2012
9	Ethoxyquin	TCVN 11283:2016 (AOAC 996.13)
11	Escherichiacoli	TCVN 7924-2:2008
12	Salmonella	TCVN 10780-1:2017 (ISO 6579-1:2017)

4. MANAGEMENT REGULATION

4.1. Declaration of conformity

Organizations and individuals shall submit declarations of conformity of feed supplements as follows:

4.1.1. Regarding domestically manufactured products, the certification result given by the certification body registered or accredited as prescribed by law shall be sent.

4.1.2. Regarding imported products, the certification or assessment result given by the certification body or the conformity assessment body registered or accredited as prescribed by law shall be sent.4.2. Conformity assessment

Organizations and individuals shall assess conformity of feed supplements as follows:

4.2.1. Regarding domestically manufactured products, Method 1 (Representative sample testing) shall be adopted.

4.2.2. Regarding imported products, Method 7 (Testing and evaluation of batches of goods) shall be adopted.

4.3. Procedures and documentation requirements for declaration of conformity are specified in Clauses 3 and 4 Article 1 of the Circular No. 02/2017/TT-BKHCN dated March 31, 2017 of the Ministry of Science and Technology on amendments to the Circular No. 28/2012/TT-BKHCN dated December 12, 2012.

Departments of Agriculture and Rural Development of provinces and central-affiliated cities shall receive documents submitted for declaration of conformity by organizations and individuals registering their manufacturing and trading within provinces and cities.

4.4. Contents, sequence and principles of using conformity assessment methods are specified in the Appendix II of the Circular No. 28/2012/TT-BKHCN dated December 12, 2012 of the Ministry of Science and Technology.

5. RESPONSIBILITIES OF ORGANIZATIONS AND INDIVIDUALS

Companies and individuals mentioned in Section 1.2 shall comply with regulations of this document.

6. IMPLEMENTATION

6.1. The Directorate of Fisheries and Departments of Agriculture and Rural Development of provinces and cities shall inspect the implementation of this document as assigned by the Ministry of Agriculture and Rural Development.

6.2. The Directorate of Fisheries shall disseminate, provide guidance and cooperate with relevant authorities in organizing the implementation of this document.

6.3. In the cases where any of the regulations specified in this document is amended or replaced, the newest one shall apply.

 

QCVN 02 - 31 - 3 : 2019/BNNPTNT

 

NATIONAL TECHNICAL REGULATION AQUACULTURE FEED

PART 3: FRESH AND LIVE FEEDS

 

Foreword

QCVN 02 - 31 - 1 : 2019/BNNPTNT is developed by Research Institute for Aquaculture No. 3, presented by the Directorate of Fisheries and promulgated by the Minister of Agriculture and Rural Development together with the Circular No. 07/2019/TT-BNNPTNT dated August 07, 2019.

 

NATIONAL TECHNICAL REGULATION AQUACULTURE FEED

PART 3: FRESH AND LIVE FEEDS

1. GENERAL

1.1. Scope

This document provides for safety criteria and maximum levels in fresh and live feeds (HS code 2309.90.90) intended for use in aquaculture.

1.2. Regulated entities

This document applies to organizations and individuals involved in manufacturing and import of fresh and live feeds intended for use in aquaculture in Vietnam.

This document does not apply to organizations and individuals that manufacture fresh and live feeds themselves for internal use.

1.3. Normative references

AOAC 986.15, Arsenic, cadmium, lead, selenium and zinc in human and pet foods by atomic absorption spectrometry (AAS) and anodic stripping voltammetry (ASV).

ISO 21872-1:2017,Part 1: Detection of potentially enteropathogenic Vibrioparahaemolyticus, Vibrio cholerae and Vibrio vulnificus.

Marteilia refringens. 2018, Manual of Diagnostic Tests for Aquatic Animals. Infection withMarteilia refringens. Part 2.2.4. OIE.

Perkinsus Olseni. 2018, Manual of Diagnostic Tests for Aquatic Animals. Infection with Perkinsus Olseni. Part 2.2.7. OIE.

Bonamia ostrea. 2018, Manual of Diagnostic Tests for Aquatic Animals. Infection with Bonamia ostreae. Part 2.2.3. OIE.

In the cases where new regulations on sampling, preparation of samples and test methods are promulgated or old ones specified in this document are amended or replaced, the newest ones shall apply.

1.4. Interpretation of terms

For the purposes of this document, the terms below shall be construed as follows:

Fresh and live feeds specified in this document refer to organisms that have not undergone any processing, are in live, fresh or frozen form and used as aquaculture feeds.

2. TECHNICAL PRESCRIPTION

2.1. Microbiological criteria:

Table 1- Maximum levels

Criterion Form	Vibrio cholera (CFU/g)	Vibrio vulnificus (CFU/g)	Vibrio parahaemolytycus (CFU/g)
Fresh and live artemia	Not specified		Not detected (in 25g)
Fresh and live bivalvia	Not detected (in 25g)	103	103

2.2. Heavy metal criteria:

Table 2- Maximum levels

Criterion Form	Pb (mg/kg)	Cd (mg/kg)
Fresh and live bivalvia	1,5	2
Fresh and live cephalopods and gastropods	1	2

2.3. Parasitic criteria:

Table 3- Maximum levels

No.	Feeds	Marteilia refringens	Perkinsus olseni	Bonamia ostreae
1	Fresh and live bivalvia	Negative

3. TEST METHODS

3.1. Sampling and preparation of test samples

TCVN 5276:1990 is applied.

3.2. Test methods

No.	Criterion	Test method
1	Pb	AOAC 986.15
2	Cadmium	AOAC 986.15
3	Vibrio parahaemolytycus, Vibrio vulnificus, Vibrio Cholera	ISO 21872-1:2017
4	Marteilia refringens	Manual of Diagnostic Tests for Aquatic Animals. Infection with Marteilia refringens. Part 2.2.4. OIE.
5	Perkinsus olseni	Manual of Diagnostic Tests for Aquatic Animals. Infection with Perkinsus Olseni. Part 2.2.7. OIE.
6	Bonamia ostreae	Manual of Diagnostic Tests for Aquatic Animals. Infection with Bonamia ostreae. Part 2.2.3. OIE.

4. MANAGEMENT REGULATION

4.1. Declaration of conformity

Organizations and individuals shall submit declarations of conformity of fresh and live feeds as follows:

4.1.1. Regarding domestically manufactured products, the certification result given by the certification body registered or accredited as prescribed by law shall be sent.

4.1.2. Regarding imported products, the certification or assessment result given by the certification body or the conformity assessment body registered or accredited as prescribed by law shall be sent.

4.2. Conformity assessment:

Organizations and individuals shall assess conformity of fresh and live feeds as follows:

4.2.1. Regarding domestically manufactured products, Method 1 (Representative sample testing) shall be adopted.

4.2.2. Regarding imported products, Method 7 (Testing and evaluation of batches of goods) shall be adopted.

4.3. Procedures and documentation requirements for declaration of conformity are specified in Clauses 3 and 4 Article 1 of the Circular No. 02/2017/TT-BKHCN dated March 31, 2017 of the Ministry of Science and Technology on amendments to the Circular No. 28/2012/TT-BKHCN dated December 12, 2012.

Departments of Agriculture and Rural Development of provinces and central-affiliated cities shall receive documents submitted for declaration of conformity by organizations and individuals registering their manufacturing and trading within provinces and cities.

4.4. Contents, sequence and principles of using conformity assessment methods are specified in the Appendix II of the Circular No. 28/2012/TT-BKHCN dated December 12, 2012 of the Ministry of Science and Technology.

5. RESPONSIBILITIES OF ORGANIZATIONS AND INDIVIDUALS

Organizations and individuals mentioned in Section 1.2 shall comply with regulations of this document.

6. IMPLEMENTATION

6.1. The Directorate of Fisheries and Departments of Agriculture and Rural Development of provinces and cities shall inspect the implementation of this document as assigned by the Ministry of Agriculture and Rural Development.

6.2. The Directorate of Fisheries shall disseminate, provide guidance and cooperate with relevant authorities in organizing the implementation of this document.

6.3. In the cases where any of the regulations specified in this document is amended or replaced, the newest one shall apply.